Mouse anti-DYKDDDDK mAb sepharose Purification kit
Affinity purified anti-DYKDDDDK antibodies, developed in mouse, were conjugated to NHS-sepharose. It is an efficient technique for isolating recombinant proteins or mammalian expression proteins. The DYKDDDDK epitope system relies on the peptide (N-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys-C), which is able to react with N- and C- terminal DYKDDDDK tagged fusion proteins and may be used for the immunoprecipitation or immune affinity purification.
The purified antibody is immobilized at 3-5 mg antibody per mL of 50% slurry
and this kit allows a rapid and efficient affinity purification of active DYKDDDDK fusion proteins. The affinity resin allows an efficient binding of DYKDDDDK fusion proteins without the need for preliminary steps and calibrations. The affinity bound DYKDDDDK fusion proteins can be efficiently eluted from the resin by acid condition. The eluted proteins can be used for characteristic analysis.
The purified antibody is immobilized at 3-5 mg antibody per mL of 50% slurry
and this kit allows a rapid and efficient affinity purification of active DYKDDDDK fusion proteins. The affinity resin allows an efficient binding of DYKDDDDK fusion proteins without the need for preliminary steps and calibrations. The affinity bound DYKDDDDK fusion proteins can be efficiently eluted from the resin by acid condition. The eluted proteins can be used for characteristic analysis.
Component:
Note: Sepharose is 1:1 suspension in Phosphate Buffered Saline, pH 7.4, containing 0.05% sodium azide as a preservative.
Product capacity:
The binding and elution capacity of 1 mL Mouse anti-DYKDDDDK mAb sepharose are commonly more than 1 mg of DYKDDDDK fusion proteins. Trying different elution buffers for optimal results is recommended.
Materials Required but Not Provided:
For sustainable use and long term storage, store at 2 °C to 8 °C. DO NOT FREEZE.
Shipping Conditions:
Blue ice
| Reagent | Quantity for 10 rxns (C07014-K02) |
Quantity for 5 rxns (C07014-K01) |
composition |
| Mouse anti-DYKDDDDK mAb sepharose | 2 mL X 1 vial | 1 mL X 1 vial | 50% slurry of Mouse anti- DYKDDDDK mAb sepharose in 1X Phosphate Buffered Saline |
| Wash Buffer (10X concentration) |
5.0 mL X 1 vial | 2.5 mL X 1 vial | 100 mM Tris/Cl pH 7.5; 1.5 M NaCl; 5 mM EDTA |
| Elution Buffer | 10 mL X 1 vial | 5 mL X 1 vial | 0.1 M Glycine pH 2.7 |
| Neutralization Buffer | 2 mL X 1 vial | 1 mL X 1 vial | 2 M Tris pH 8.0 |
| spin column | 10 pcs | 5 pcs | |
| collection tube | 10 tubes | 5 tubes |
Product capacity:
The binding and elution capacity of 1 mL Mouse anti-DYKDDDDK mAb sepharose are commonly more than 1 mg of DYKDDDDK fusion proteins. Trying different elution buffers for optimal results is recommended.
Materials Required but Not Provided:
- Micro-centrifuge capable of 15,000 x g
- 1.5 mL Centrifuge tubes
- End-over-end rotator
- CoIP Lysis Buffer (mild reaction): 10 mM Tris/Cl pH 7.5; 150 mM NaCl; 0.5 mM EDTA; 0.5% NP-40
- RIPA (vigorous reaction): 100 mM Tris/Cl pH 7.5; 300 mM NaCl; 0.2% Sodium Deoxycholate (or 0.1% SDS); 2% NP-40
For sustainable use and long term storage, store at 2 °C to 8 °C. DO NOT FREEZE.
Shipping Conditions:
Blue ice
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